| Customization: | Available |
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| CAS No.: | 77-86-1 |
| Formula: | N/a |
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TRIS, also known as Tris(hydroxymethyl)aminomethane, is a widely used chemical in biological and medical applications due to its buffering properties. Here are some of its primary uses:
Biochemical Buffer: TRIS is commonly used as a buffer in various biological experiments to maintain a stable pH environment. It is particularly effective in the pH range of 7.0 to 9.0 and is a component of common buffers like TAE and TBE used in DNA and RNA research.
Molecular Biology: It serves as a solvent for nucleic acids and proteins, often used in the preparation of solutions for DNA and RNA extraction, as well as in the buffers for enzymes and other biological macromolecules.
Electrophoresis: TRIS is a key component in the buffer systems used for protein and nucleic acid electrophoresis, helping to maintain a stable pH during the process.
Crystallography: It is used in the growth of protein crystals, a technique important for structural studies in biochemistry.
Cell Culture: TRIS is sometimes used in cell culture media to provide a stable environment for cell growth.
Pharmaceuticals: It has applications in the pharmaceutical industry, including as a component in some medications.
Chemical Synthesis: TRIS acts as an intermediate in the synthesis of other chemicals, including surface active agents and pharmaceuticals.
Acid-Base Regulation: In medical contexts, it has been used to treat acidosis by acting as a buffer in the body.
Research: It is utilized in various research applications where precise pH control is necessary.
TRIS is a versatile compound due to its ability to buffer solutions over a range of pH values, making it indispensable in many laboratory settings. It is important to handle TRIS with care, as it can be corrosive and may have other hazardous effects.
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