Nitrotetrazolium Blue Chloride CAS 298-83-9 Health Care High Quality

• Customization: Available
• Transport Package: Drum
• Specification: 99%
• Model NO.: 298-83-9
• Trademark: PUYER
• Origin: China
• Production Capacity: 10000kg/Month

Product Description

Product Description

 

Nitrotetrazolium Blue chloride (NBT) is a yellow crystalline powder that is soluble in water and methanol. It is commonly used as a redox indicator in biological experiments. When reduced, it forms a purple formazan product, which is insoluble in water. NBT is often used in conjunction with 5-bromo-4-chloro-3-indolyl phosphate (BCIP) as a substrate for alkaline phosphatase, resulting in a blue-colored product that is insoluble in water. This makes it a popular choice for various staining procedures in biological research.

NBT is used in several applications, including:

1. As a substrate for detecting dehydrogenases and other oxidoreductases.
2. In the determination of glucose-6-phosphate dehydrogenase activity.
3. As a precipitating agent for fibrinogen.
4. In immunohistochemistry (IHC), immunocytochemistry (ICC), and Western blotting (WB) to visualize the presence of specific proteins.
5. As a screening method for urinary tract infections.
6. In the detection of serum glycosylated low-density lipoprotein and very-low-density lipoprotein, where the degree of color development is indicative of the concentration of these lipoproteins.

The chemical formula for NBT is C40H30N10O6·2Cl, with a molecular weight of 817.60 and a CAS number of 298-83-9. It is important to handle NBT with care due to its toxic nature and potential carcinogenicity. It should be stored at 2-8°C, protected from light, and disposed of according to proper laboratory safety protocols.

 

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