| Customization: | Available |
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| Transport Package: | Drum |
| Specification: | 99% |
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Ferrozine sodium, also known as Ferrozine, is a chemical compound widely used as a spectrophotometric reagent for the detection and quantification of iron, particularly ferrous ions (Fe^2+). It forms a stable, intense magenta-colored complex with Fe^2+, which has an absorption peak at 562 nm, making it a useful tool in various analytical procedures.
Ferrozine reacts with ferrous iron (Fe^2+) to form a colored complex. This complexation reaction is the basis for its use in colorimetric assays to quantify iron levels in different samples, including biological systems. The reaction is highly specific for Fe^2+ and is not significantly affected by other divalent metal cations.
Ferrozine is commonly used in biological research to measure iron levels in cultured cells. It has been utilized in assays to study iron accumulation in cells, such as brain astrocytes. The assay involves treating cell lysates with an acidic KMnO4 solution to make iron complexed to cellular proteins accessible to ferrozine. This method has been found to provide accurate and reliable measurements of intracellular iron content, comparable to those obtained by atomic absorption spectroscopy .
Ferrozine should be stored at 4°C, sealed, and protected from moisture and light. When dissolved in a solvent, it can be stored at -80°C for up to 6 months or at -20°C for up to 1 month
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