| Customization: | Available |
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| Transport Package: | Cartons/Drums |
| Specification: | drum |
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Potassium alpha-ketoisocaproate (KIC) is recognized as an important intermediate in the metabolism of the branched-chain amino acid leucine. It has various applications, particularly as a nitrogen-free substitute for leucine in therapeutic diets for patients with chronic kidney disease and hepatitis B virus infection, helping to meet their daily leucine requirements while reducing nitrogenous waste .
The biosynthesis of KIC can be achieved through different pathways. One approach involves using glucose as a substrate and metabolically engineered strains of Corynebacterium glutamicum or recombinant Escherichia coli, although this typically results in lower yields . Another method is enzymatic catalysis using amino acid aminotransferases, oxidases, or deaminases, along with whole-cell bioconversion with recombinant engineering strains and L-leucine as a substrate, which tends to yield higher concentrations of KIC .
Research has also focused on developing whole-cell biocatalysts for the production of KIC. For example, one study achieved one-step biosynthesis of KIC from L-leucine using an E. coli whole-cell biocatalyst expressing an L-amino acid deaminase from Proteus vulgaris. This method optimizes the reaction conditions for bioconversion and examines various L-leucine supply strategies to enhance KIC production .
In terms of environmental and industrial benefits, the biotransformation process for KIC production is considered more environmentally friendly compared to chemical synthesis methods, which can be costly and result in environmental pollution. Microbial production of KIC offers advantages such as high yield, productivity, and flexibility in substrate consumption .
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