| Customization: | Available |
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| Transport Package: | Cartons/Drums |
| Specification: | drum |
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Sodium alpha-ketoisocaproate (KIC) is recognized as an important intermediate in the metabolism of the branched-chain amino acid leucine. It has applications as a nitrogen-free substitute for leucine in therapeutic diets for patients with chronic kidney disease and hepatitis B virus infection, helping to meet their daily leucine requirements while reducing nitrogenous waste .
The biosynthesis of KIC can be achieved through various methods, including chemical synthesis and biotransformation. Chemical synthesis methods, such as the azlactones method, Grignard reagents with diethyloxamates, and the hydantoin method, are commonly used but can be costly and require expensive catalysts or special starting structures .
In contrast, biotransformation offers an environmentally friendly process for KIC production, which is more attractive for industrial production. This method can be achieved through microbial fermentation or enzymatic transformation. For instance, a recombinant Corynebacterium glutamicum strain was engineered to develop a fermentation process, reaching a maximum KIC titer of 9.23 g/L . Another approach involves the use of an E. coli whole-cell biocatalyst expressing an L-amino acid deaminase from Proteus vulgaris, which can convert L-leucine to KIC in a one-step biosynthesis .
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